Transactions on Additive Manufacturing Meets Medicine
Vol 1 No 1 (2019): Trans. AMMM
https://doi.org/10.18416/AMMM.2019.1909S03P19

Imaging and Modelling in 3D Printing

Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT

Main Article Content

Tabea Kohlfaerber (Medizinisches Laserzentrum Lübeck GmbH), Shujun Ding (Institute of Biomedical Optics, Universität zu Lübeck, Germany), Ramtin Rahmanzadeh (Institute of Biomedical Optics, Universität zu Lübeck, Germany), Thomas Jüngst (Department for Functional Materials in Medicine and Dentistry (FMZ), University Hospital of Würzburg, Germany), Jürgen Groll (Department for Functional Materials in Medicine and Dentistry (FMZ), University Hospital of Würzburg, Germany), Hinnerk Schulz-Hildebrandt (Medizinisches Laserzentrum Lübeck GmbH, Lübeck, Germany; Institute of Biomedical Optics, Universität zu Lübeck, Germany; Airway Research Center North (ARCN), Member of the German Center of Lung Research (DZL), Gießen, Germany), Gereon Hüttmann (Medizinisches Laserzentrum Lübeck GmbH, Lübeck, Germany; Institute of Biomedical Optics, Universität zu Lübeck, Germany; Airway Research Center North (ARCN), Member of the German Center of Lung Research (DZL), Gießen, Germany)

Abstract

Optical coherence tomography is a non-invasive and label-free imaging modality based on the detection of backscattered light in samples. As microscopic OCT (mOCT) combines high axial and lateral resolution, an investigation of biological and printed samples at subcellular level is feasible.  The additional excellent depth resolution enables mOCT to be a suitable quality control of 3D printed samples. The use of speckle variance adds information about cell viability. Here we present the feasibility of investigating cell viability within a cell spheroid and monitoring the cell interaction with bioprinted scaffolds using mOCT.

Article Details